Articles

Experimental Reports:

Experimental Report 5:

Measuring the Life Energy Phenomena in a Yeast Culture

by Heliognosis

Background

Until now the only way to determine if cells are alive or dead is by using subjective observations such as movement, the presence of metabolism products, consumption of media, production of gasses etc. The vigor and health of cells can only be indirectly measured. Wilhelm Reich illustrated in the Bion Experiments and later in The Cancer Biopathy that cell vigor could be monitored using special techniques such as dark-field microscopy. Using apochromatic objectives, the blue energetic glow of healthy cells could be seen directly at high magnification. Similarly, Reich treated human blood with a salt solution and observed the length of time before cell death using the dark-field technique. By careful observation of the cell contraction and eventual time of cell membrane breakage, he was able to determine the overall energetic strength of the blood cells and of the person from whom they were taken. In this report a method for monitoring cell culture growth and cell vitality is presented using the Heliognosis LM3 Experimental Life Energy Meter in conjunction with a liquid/cell culture probe.

Life Energy Meter Fluid Probe System reference solution and culture
Fluid Probe Apparatus and LM3 Reference solution and culture

Method:

The Heliognosis Experimental Life Energy Meter Model LM3 Rev B. was placed on a frame with the reference and sample liquid electrodes mounted behind. The apparatus was positioned on a wooden table with all other objects moved at least 18" away. Two test tube solutions were prepared, each containing exactly 10ml of pure water1. The two tubes were screwed into the reference and sample electrodes. The calibration adjustment was carefully rotated until both the reference and sample electrode read exactly 0% on the x100 range of the Life Meter. To the sample tube was added 0.8g of Lantic white granulated sugar. The meter now read 9%. The sample tube was then unscrewed and 0.2g of Baker's yeast (Fleischmann's) was added. The tube was immediately screwed into the sample electrode and the timer started.

Observations:

Immediately upon re-attaching the sample electrode with the yeast solution, the reading was 12%. The yeast initially sank to the bottom of the tube. A cloudiness gradually began to form and was distinct after 13 minutes. After 20 minutes, bubbling began from the bottom of the solution. The room temperature was 19.5 degrees C.

When the yeast culture had been growing for 33 minutes, it was observed that the culture was rising to the top of the solution and a gas filled foam developed. At this point the readings on the meter, that had been increasing gradually, began to climb more rapidly. The reference electrode was periodically checked using the switch on the liquid probe to make sure that the instrument was still calibrated. If the reference had deviated away from zero, the Life Meter zero was adjusted to keep the readings accurate. From 40 minutes onward the surface culture continued to grow finally to a thickness of 7mm between 94 and 135 minutes. Gasses and culture solution would occasionally overflow from the vent hole as the growth progressed. Excess was wiped away to prevent it from altering the reading of the inside culture growth. After 135 minutes, the evolution of gas and the rate of culture growth dropped rapidly with the collapse of the surface culture. The deposit of yeast at the bottom of the tube was now much smaller. Bubbling continued from below but at a much lower rate.The culture's activity became insignificant beyond 325 minutes with only occassional bubbles rising from the bottom. Below is shown a picture of the cell culture after 104 minutes. The Life Meter data is plotted graphically below and shows the detected energy level of the culture over time

yeast culture on solution surface energy level of yeast culture over time
Yeast culture 
on solution surface
Detected energy level 
of yeast culture over time

Conclusion:

The visual observations of culture growth and decline matched perfectly with the data recorded from the Life Meter. During their life cycle, the cells accumulated energy and then released it again to the environment. It is now apparent that the displacement currents utilized by the Life Meter can detect the Life energy present in these cells and can monitor its increase and decrease over the cells life cycle.This may lead to the possibility of differential diagnosis of cell health. 

Notes

1.Luso bottled water, containing 41.6 ppm of dissolved salts